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Item #:
84926563
Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade
86% of respondents would recommend this to a friend
Item #:
84926563
XCD 81
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Ready-to-use DPBS solution that does not contain calcium or magnesium, suitable for general use in the laboratory.
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What Stands Out
Ideal Buffering
Formulated for optimal pH 7.4 stability, ensuring reliable performance in cell culture and laboratory experiments, promoting reproducible results across various biological applications.
Calcium/Magnesium Free
Excludes calcium and magnesium, preventing unintended cellular responses, making it perfect for experiments requiring precise control over ionic conditions, enhancing protocol accuracy.
Convenient Volume
Comes in a 500 mL bottle, providing ample solution for extensive lab use while ensuring fresh, high-quality reagent specifically designed for continuous lab-grade applications.
Product Details
| Package Weight | 2.53 Pound |
Who Should Buy?
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Cell Culture
Ideal for maintaining and washing mammalian cells in culture due to its isotonic and buffered nature.
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Biological Experiments
Suitable for use in various biological experiments requiring a stable pH and saline environment without calcium or magnesium.
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Laboratory Research
A dependable reagent for researchers needing a standard buffer solution for their laboratory needs in various assays.
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In Vivo Use
Not suitable for in vivo applications as it lacks nutrients and necessary components for living organisms.
Product Description
Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is Dulbecco's Phosphate Buffered Saline (DPBS)?
Answer: Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution commonly used in laboratory settings to maintain physiological pH and osmotic balance. This specific formulation is designed without calcium and magnesium, making it ideal for cell culture work where those ions could interfere with certain cell lines. Researchers often use DPBS for washing cells, diluting samples, or reconstituting cells in culture, ensuring that the environment remains stable for optimal cell growth. -
Question:
Why is calcium and magnesium omitted from this DPBS formulation?
Answer: Calcium and magnesium are omitted from this formulation to prevent potential cell aggregation or clumping, which can occur during certain biological procedures. This makes Dulbecco's Phosphate Buffered Saline Without Calcium and Magnesium particularly suitable for cell types that require specific handling conditions or for experiments that necessitate strict control of the ionic environment. It effectively ensures that cells can be manipulated without the risk of unwanted interactions. -
Question:
What is the pH level of this solution and why is it important?
Answer: The pH level of this Dulbecco's Phosphate Buffered Saline is precisely maintained at 7.4. This neutral pH is critical as it closely mimics the physiological pH of human blood, offering an optimal environment for cell culture and biological assays. Maintaining a consistent pH helps prevent cellular stress or changes in cellular behavior, which can significantly alter the outcomes of experimental results. -
Question:
In what applications is DPBS typically used?
Answer: DPBS is widely utilized in various applications including cell culture, tissue storage, and as a diluent for other reagents. It serves as an excellent wash solution for cells before transferring them into fresh media, helping to remove any residual serum or substances that could affect experimental outcomes. Researchers also use it for diluting solutions during immunoassays or other biochemical analyses, ensuring optimal conditions for their specific applications. -
Question:
How should Dulbecco's Phosphate Buffered Saline be stored?
Answer: Dulbecco's Phosphate Buffered Saline should be stored at 2-8 degrees Celsius, away from light to preserve its quality. For long-term storage, the solution can be kept in refrigerated conditions, but it is essential to avoid freezing, which could compromise its integrity. Proper storage prevents microbial contamination and maintains the stability of the buffer, thus ensuring consistent performance in laboratory applications. -
Question:
Is Dulbecco's Phosphate Buffered Saline suitable for all cell types?
Answer: While DPBS is versatile and used for various cell types, it is crucial to verify that the specific cell line being used does not have special requirements for ions like calcium or magnesium. Certain cell types may depend on these ions for attachment or growth, so it's essential to consult specific protocols for those cell lines. However, for many standard applications, DPBS is an excellent choice for maintaining cell viability and functionality. -
Question:
Can you use Dulbecco's Phosphate Buffered Saline directly from the bottle?
Answer: Generally, it's advisable to transfer an appropriate amount of DPBS into a sterile container when using it for cell cultures or experimental procedures. This practice minimizes the risk of contamination as touching or repeatedly opening the original bottle can introduce microorganisms. Additionally, having a portion in a separate sterile container allows for more controlled and accurate volumes when performing various experimental tasks. -
Question:
What are the safety considerations when using DPBS?
Answer: Dulbecco's Phosphate Buffered Saline is considered safe for laboratory use; however, standard laboratory safety practices should always be followed. It's important to use personal protective equipment, such as gloves and goggles, when handling the solution. Additionally, always dispose of any unused or expired DPBS according to your local regulations for biological waste, ensuring a safe laboratory environment for all personnel. -
Question:
Can DPBS be used for in vitro experiments?
Answer: Yes, Dulbecco's Phosphate Buffered Saline is widely utilized in in vitro experiments due to its ability to closely mimic physiological conditions. It provides a stable environment without interfering chemicals, making it ideal for assays, cell culture, and various other biological experiments. Whether you are culturing eukaryotic or prokaryotic cells, using DPBS helps maintain consistency and reliability in experimental results. -
Question:
Where can I buy Dulbecco's Phosphate Buffered Saline 1X DPBS in Anguilla?
Answer: You can buy Dulbecco's Phosphate Buffered Saline 1X DPBS, Without Calcium and Magnesium, pH 7.4, 500 mL, Lab Grade at Ubuy in Anguilla. Ubuy offers a convenient shopping experience with a variety of lab supplies, and you can be assured of quality products to meet your laboratory needs.
Albert Bio Buffers Editorial Review
Dulbecco's Phosphate Buffered Saline (1X DPBS), Without Calcium and Magnesium, pH 7.4, 500 mL, is an essential lab-grade solution provided by Albert Bio, designed to maintain cell viability and physiological conditions for biological experiments. The solution comes in a convenient 500 ml bottle, making it easy to measure and use for various applications such as cell culture and molecular biology. Users appreciate its reliable quality and optimal pH balance for various laboratory needs, ensuring it supports a range of experiments efficiently. This product is presented in a sturdy bottle, enhancing usability and storage in lab environments.
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Pros
- Lab grade quality for reliable results
- Optimal pH for physiological conditions
- Convenient 500 ml bottle size
- Suitable for cell culture applications
- Easy to use for molecular biology experiments
Cons
- Limited to specific applications in research labs
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XCD 81
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Features & Benefits
- Ready-to-use DPBS solution
- Does not contain calcium or magnesium
- Easy to handle
- Suitable for general use in the laboratory (not sterile)
- Made in USA
- Concentration: 137 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4
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